BioMagicTM Product Questions

1. How do BioMagicTM Gel-Free Cloning Kits work?
Conventional clonings rely on agarose gel to separate fragments
physically.  Instead, our innovative gel-free kits "separate" fragments
chemically.  The gel-free goal is achieved by a proprietary "tagging"
process.  After "tagging", only fragments of interest are suitable for
cloning reaction.  The "tagging" process is a single step 10-60 minutes
incubation after your restriction digestion.  Absolutely no PCR is
involved.

2. Do BioMagic Kits really work?  What should I do if I try them
without success?
Yes, our kits absolutely work!  Make sure order the right kit for your
cloning!
If you fail, keep in mind that 1) there are slew of things can go wrong
other than our kits.  2) The only step introduced by our kits is the 10-60
minutes incubation ("tagging process).  The tagging process is highly
reliable with guaranteed accuracy and efficiency.  
If something goes wrong, more likely It is "outside" our kits.  Please click
on our troubleshooting page.  We provide comprehensive solutions to
your cloning problems.

3. What's the protocol for your kits?
Step 1 restriction digestion
Step 2 10-60 minutes incubation (new)
Step 3 inactivation of restriction enzymes
Step 4 ligation
Step 5 transformation

There is only one single incubation step introduced!  However, gel
electrophoresis and gel purification are completely eliminated.

4. Why BioMagicTM Cloning kits have much higher cloning
efficiency comparing with conventional gel cloning approach?
The reason is simple: there is absolutely no UV/EtBr damage to DNAs
with our BioMagic Gel-Free kits!

5. Is it true that I can use BioMagicTM Gel-Free Cloning Kit (II) for
clonings without compatible ends avoiding PCR or
mutagenesis?
Yes, that's what our Biomagic all about!  



            BioEfficient Cloning Service Questions

1. How do I order your services?
Step 1 - Simply send us an email or fill out the Form with the description
of your project on our Price and Order page.
Step 2  - We send you a price quote.
Step 3 - Issue us a PO number or credit card information.
Step 4 - Send us your starting material if any.

2. How much does it cost for a subcloning?
For high-throughput cloning including TA cloning, prices are in the tens
of dollars.  For customized subcloning, prices start in the few hundred
of dollars.  Significant discounts apply for larger orders whether in a
single PO or over a period of time.

Please contact us for your quote.

3. What is the turn-around time?
We normally complete a one ligation-only subcloning in 10-14 days.
Depending upon our client's time frame along with the level of difficulty,
the time could be shorter or longer.

4. What does BioEfficiency provide as product?
We can provide product as requested.  Normally we provide 1-5 ug
minipreps and cell stabs of constructs, along with any verification data.

5. How are results verified?
Either by restriction digestion or sequencing as needed.

6. Will you be able to provide final construct if I have nothing but a
paper sequence and design of the final construct?
Yes, we will be able to provide you with desired construct.  If you have
more materials to start with, please go on to the next question/answer.

7. What materials should I provide?
1. If no PCR needed
a) 50ug of each parental plasmid and destination
vector with concentration
b) both maps and sequences of parental plasmid and
destination vector or specify the commercial sources

2. If PCR or RT-PCR needed.  Please add:
a) 20ug template labeled with concentration or 3
RNA samples with proof of quality and quantity
for RT-PCR
b) 100ul of 10uM primers if available
c) primer sequences/Tm (if available) and gene
sequence to be amplified by email
d) sequencing primers if applicable and available

                        General Questions

1. My project is confidential; how do you handle this work?
We can sign a confidentiality agreement upon request.

2. Methods of payment?
We accept purchase orders and major credit cards.

FAQ