BioMagic Cloning Products

BioMagicTM Cloning Products allow you to clone smart not hard!
Our innovative BioMagicTM Gel-Free Cloning Kits rely on our proprietary Gel-Free Technology.
We developed a mix of proprietary chemicals, polymers, proteins and etc to specifically "tag"
fragments of interest for cloning. The "tag" is based on certain cloning sites, length, adjacent
bases, GC content, secondary structure and etc. After being "tagged", only fragments of
interest are suitable for cloning reaction. In the meanwhile, undesired fragments become
supercoiled at various levels. The necessity of gel to separate the desired from the undesired is
eliminated. Additionally, our products solve another common problem: cloning fragments
inseparable from multiple similar-size fragments on gel generated due to no or minimal size
difference. Our kits include;

BioMagicTM Gel-FreeTA Cloning Kit
BioMagicTM Gel-Free PCR Cloning Kit
BioMagicTM Gel-Free Cloning Kit I (compatible cut/paste cloning)
BioMagicTM Gel-Free Cloning Kit II (incompatible cut/paste cloning)
BioMagicTM Loading Buffer Identify recombinant with our single buffer in 10 minutes!

BioMagicTM Cloning Kit I (for cut/paste cloning with compatible ends)

Advantages:

Eliminate gel electropherosis and gel purification completely during cloning
Ultimate cloning solution for insert inseparable from multiple fragments generated by
restriction digestion
Universal/works with any vector/insert of customer's choice
Only nanogram quantities of DNAs required
Only 6 minutes ligation at room temperature
Higher cloning efficiency due to EtBr/UV damage free
Environmentally friendly

Application

Cloning of any insert/vector of customer's choice with compatible ends

Content

BioMagicTM V.I.P.TM Solutions
BioMagicTM ligase and buffer

Protocol

Restriction digestion
Incubation with V.I.P solutions for 30-60 minutes
Inactivate restriction enzymes
Ligation
Transformation
Screen 4 clones for recombinants

Place Order

BioMagicTM Cloning Kit II ( for cut/paste cloning without compatible ends)

Advantage

Eliminate PCR or mutagenesis to create compatible ends
Completely eliminate gel electropherosis and gel purification during cloning
Ultimate cloning solution for insert inseparable from multiple fragments generated by
restriction digestion
Only nanogram quantities of DNAs required
Only 6 minutes ligation at room temperature
Environmentally friendly
Higher cloning efficiency due to EtBr/UV damage free

Application

Cloning of insert/vector of customer's choice without compatible ends

Content

BioMagic V.I.P.(II) solutions
BioMagic ligase and buffer

Protocol

Restriction digestion
Incubation with V.I.P.(II) for 10-60 minutes
Inactivate restriction enzymes
Ligation
Transformation
Screen 4 clones for recombinants

Place Order

BioMagicTM TA Cloning Kit

*specific PCR product is prerequisite/essential

Advantage

Works with any fragment, with or without 3' A overhang
Eliminate gel electropherosis and gel purification during TA cloning and insert shuttling
Not limited by inseparable inserts from multiple fragments generated by restriction
digestion
Only nanogram quantities of DNAs required
Lower price compared to other vendors
Only 6 minutes TA reaction at room temperature
Higher cloning efficiency due to EtBr/UV damage free
Environmentally friendly

Application

Cloning of fragment with or without 3' A overhang into BioMagic TA vector
high throughput format is available

Content

BioMagicTM TA vector
BioMagic V.I.P.(TA) Solutions

Protocol

Set up TA reaction directly if fragment has 3' A overhang
Incubate with V.I.P.(TA) solutions for 10 minutes if no 3' A overhang before TA reaction
When shuttle gene fromTA vector to destination vector of your choice, add V.I.P.(TA)
solutions to restriction digestion mixture.
Ligation
Transformation
Screen 4 clones for recombinant

Place Order

BioMagicTM PCR Cloning Kit

*specific PCR product required

Advantages

Eliminate PCR purification column, gel electropherosis, and gel purification for cloning
PCR fragment when clean/specific PCR product available
Direct digestion of PCR reaction followed by direct cloning without purification
Universal - Works with any vector/insert of customer's choice
Only nanogram quantities of DNAs are required
Only 6 minutes ligation at room temperature
Environmentally friendly
Higher cloning efficiency due to EtBr/UV damage free

Application

Cloning PCR fragment with or without 3' A overhang into vector of customer's choice
High throughput format is available

Content

BioMagic V.I.P.(PCR) solution
BioMagic column
BioMagc ligase and buffer

Protocol

Add V.I.P.(PCR) solutions to PCR reaction if template is plasmid DNA
Or pass through BioMagic column if template is genomic DNA or any large size DNA
Restriction digestion
Ligation at room temperature for 6 minutes

BioMagicTM Loading Buffer

Identify recombinants directly with a single BioMagic Loading Buffer in 10 minutes!

Advantage

Directly add loading buffer to colonies from plate and immediately resolve on gel on gel for
recombinant clone
Tremendous savings in time, cost and labor
Very low background
More reliable than colony PCR screening

Application

Screen recombinant clones in 10 minutes with single buffer

Content

BioMagic loading buffer clear
BioMagic loading buffer blue

Protocol

Mix clear and blue solution to make final BioMagic loading buffer
Pick up single colony
Add 10ul BioMagic loading buffer (10ul to 1ul >50ng circular vector as control)
Vortex 10 seconds
Resolve on 0.8%-1% agarose gel for 5 minutes
Soak in EtBr for 5 minutes
Potential recombinant clones are larger than the control

Place Order

	Cloning Hard	Cloning Smart
Digestion	Yes	Yes
Gel Electropherosis	Yes	No
Gel Purification	Yes	No
Ligation	Hours or overnight at 16C	6 mins at room temperature

	Cloning Hard	Cloning Smart
Engineering compatible ends by PCR or mutagenesis	Yes	No
Clean PCR reaction with column	Yes	No
Gel electropherosis	Yes	No
Gel purification	Yes	No
TA cloning	Yes	No
Sequencing verification of PCR fragment	Yes	No
Mutagenesis to correct PCR error	Yes	No
Digestion	Yes	Yes
Gel electropherosis	Yes	No
Gel purification	Yes	No
Ligation	Hours or overnight at 16c	6 mins at room temperature

	Cloning Hard	Cloning Smart
Cloning fragment without 3'A overhang	No	Yes
Limited by fragment size >3-4kb	Yes	No
Gel purification PCR fragment before TA cloning	Yes	No*
or clean PCR product by column	Yes	No
Enzyme limitation during shuttling gene from TA vector to customers'vector	Yes	No

Gel electrophoresis/purification during shuttling	Yes	No
Cost	$20/reaction	$14/reaction

	Cloning Hard	Cloning Smart
Clean PCR with column	Yes	No
Gel electropherosis	Yes	No*
Gel purification	Yes	No
Digestion	Yes	Yes
Ligation	Hours or overnight at 16c	6 mins at room temperature

	Clone hard	Clone Smart
Pick colonies	Yes	Yes
Grow overnight culture	Yes	No
Plasmid preparation	Yes	No
Digestion	Yes	No
Gel electropherosis	Yes	Yes
Time needed for identifying recombinant clones	2 days	10 minutes